The predictive effect of ACAA2 and ARID2 gene expressions on oocyte and embryo development

To acquiring high-quality embryos is crucial to the improvement of pregnancy rates for assisted reproductive technology (ART). But how to find a non-invasive approach to early embryo selection and improve the implantation rates from proteins in cumulus cells? Our previously study showed that acetyl-CoA acyltransferase 2 (ACAA2) protein and adenine-thymine (AT)-rich interactive domain-containing protein 2 (ARID2) protein were expressed obviously differentially between mature cumulus cells and immature cumulus cells based on proteomics technique. Furthermore, real-time polymerase chain reaction (PCR) was needed to verify the expressions of the two genes in the mature cumulus cells and the relationship between gene expression and the developmental ability of oocytes and subsequent embryos. Then, the effects of ACAA2 and ARID2 expressions on good quality embryo formation were discussed. Finally, we found that the expression of ARID2 and ACAA2 in the mature cumulus cells was significantly higher than in immature cumulus cells and was positively correlated with the rate of high-quality embryos. So, we drew the conclusions that high expression of ARID2 and ACAA2 may predict the developmental potential of oocytes and thus become predictive biomarkers for embryo quality.